Detection of New Staphylococcal Enterotoxin Encoding Genes in Staphylococcus aureus Isolated from Animals and Humans in Yogyakarta

Abstract Staphylococcal enterotoxins (SE) are essential in human and animal infection food poisoning. This study analyzed ten genes ( sek, sel, sem, sen, seo, sep, seq, ser, ses , set ) encoding new staphylococcal enterotoxin isolated from animals humans by multiplex polymerase chain reaction (PCR). In Yogyakarta, Indonesia, samples cases (174 isolates), cattle (5 goats isolates) resulted a total of 183 Staphylococcus aureus isolates.. All isolates were confirmed to be based on bacterial culture biochemistry as well identification 23S rRNA. The sel gene was most often observed (4 isolates, 80%), followed the 20% sek gene. Isolates cattle, found 44% 22%, sep 22% ser 11%. with sem (38 21% genes, 14% 13% 8% sen 2% genes. frequently included gene, whereas those contained Detecting type SE among indicates public health threat due infection. occurrence this might used an approach for controlling infections poisoning diseases.